Thermal Cycler | |
Base thermal cycler |
C1000 |
Max ramp rate |
5¡ÆC/sec |
Ave ramp rate |
3.3¡ÆC/sec |
Heating and cooling method |
Peltier |
Lid |
Heats up to 105¡ÆC |
Temperature |
|
Range |
0–100¡ÆC |
Accuracy |
¡¾0.2¡ÆC within programmed target at 90¡ÆC |
Uniformity |
¡¾0.4¡ÆC within 10 sec of arrival at 90¡ÆC |
Gradient |
Yes |
Operational range |
30–100¡ÆC |
Programmable span |
1–24¡ÆC |
Optical Detection | |
Excitation |
6 filtered LEDs |
Detection |
6 filtered photodiodes |
Range of excitation/emission |
450–730 nm |
Sensitivity |
Detects one copy of genomic DNA |
Dynamic range |
10 orders of magnitude |
Scan time — all channels |
12 sec |
Scan time — fast |
3 sec |
Software | |
Operating systems |
Windows XP Professional, Windows Vista Professional |
Multiplex analysis |
Up to 5 targets per well |
Data analysis modes |
PCR quantitation with standard curve and melt-curve analyses; gene expression analysis by relative quantity (¥ÄCT) or normalized expression (¥Ä¥ÄCT) with multiple reference genes and reaction efficiencies; multiple file gene expression analysis; allelic discrimination and end-point analyses |
Data export |
Save, copy, and print all graphs and spreadsheets from right-click menu. Export results to Microsoft Excel. Copy and paste directly into Microsoft Excel, Word, or PowerPoint files. |
System | |
Licensed for real-time PCR |
Yes |
Sample capacity |
96 wells |
Sample size |
1–50 µl (recommend 10–25 µl) |
Communications |
USB 2.0 |
Electrical approvals |
IEC, CE |
Dimensions |
33 x 46 x 36 cm (13 x 18 x 14") |
Weight |
21.4 kg (47 lb) |
Channel |
Excitation (nm) |
Detection (nm) |
Calibrated Fluorophores |
1 |
450-490 |
515-530 |
FAM¢â, SYBR Green I¢â |
2 |
515-535 |
560-580 |
VIC¢ç, HEX¢â, TET¢â, Cal Gold 540¢â |
3 |
560-590 |
610-650 |
ROX¢â, TEXAS RED¢ç, Cal Red 610¢â |
4 |
620-650 |
675-690 |
CY5, Quasar 670¢â |
5 |
672-684 |
705-730 |
Quasar 705¢â |
6 |
450-490 |
560-580 |
None (accommodates FRET Chemistry) |
CFX96 filters and factory calibrated fluorophores
°¢ ä³Î°£¿¡ ÀÚÁÖ »ç¿ëµÇ´Â Çü±¤dyeµéÀ»CFX96¿¡¼ detectionÇÑ °ªÀÔ´Ï´Ù. ä³Î °£ Çü±¤ °£¼·ÀÌ °ÅÀÇ ¾øÀ½À» º¸¿©ÁÖ´Â µ¥ÀÌÅÍÀÔ´Ï´Ù.
Accurate quantitation is accomplished across a large dynamic range. Plasmid template was diluted from 1010 copies down to 1 copy and amplified using iQ supermix in the presence of a FAM-labeled probe. Data are presented for 6 replicates per dilution.
CFX96ÀÇ High performance 1 : Linearity and Uniformity
1010°³ºÎÅÍ 1°³±îÁö plasmid DNA¸¦ 10¹è ´ÜÀ§·Î Èñ¼®ÇÏ¿© template·Î »ç¿ëÇϰí, FAM-labeled probe¸¦ ÀÌ¿ëÇÏ¿© detectionÇÑ °á°úÀÌ´Ù. °¢ dilution-factor³»¿¡¼´Â 6¹ø ¹Ýº¹½ÇÇèÀ» ÇÏ¿´´Ù. CFX96À» »ç¿ëÇϸé ÃÖ°íÀÇ Á¤·®Àû µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ÀÖÀ½À» º¸¿©ÁÖ´Â °á°úÀÌ´Ù.
CFX96ÀÇ High performance 2 : true 5 target multiplexing °á°ú
Linearity of five-target detection.: 108ºÎÅÍ 102°³ ±îÁö plasmid DNA¸¦ 10¹è ´ÜÀ§·Î Èñ¼®ÇÏ¿© template·Î »ç¿ëÇϰí, °¢°¢ÀÇ targetÀ» °¢±â ´Ù¸¥ Çü±¤¹°Áú·Î probe¸¦ ¸¸µé¾î (FAM/actin, Hex/GAPDH, Texas Red/Cyclophilin, Cy5/Tubulin and Quasar705/IL1-bB) Realtime qPCR standard curve ½ÇÇèÀ» ÇÑ µ¥ÀÌÅÍÀÌ´Ù. ¸ðµç standard curveÀÇ ¹ÝÀÀ È¿À²(reaction efficiency)Àº 97%~103% ¾ÈÀ¸·Î µé¾î¿ÈÀ» º¼ ¼ö ÀÖ´Ù. À̷μ CFX96ÀÇ ¶Ù¾î´Â ¹ÝÀÀ¼ºÀ» º¸¿©Áܰú µ¿½Ã¿¡ ½ÇÁ¦ 5 target multiplex °á°ú¸¦ ¸¸µé¾î ³¾ ¼ö ÀÖ´Â ÀåºñÀÓÀ» ÀÔÁõÇϰí ÀÖ´Ù.
Maximum VS Average Ramping Rate.
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1000 series
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Aȍ
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Eȍ
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*1000-series°¡ º¸¿©ÁÖ´Â ÁöÁ¤¿Âµµ·ÎÀÇ ºü¸¥ µµÂø ¹× ¶Ù¾î³ heating & cooling ½ÃÀÇ uniformity |
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*ÀÌ¿Í °°ÀÌ ¶Ù¾î³ Uniformity ¹× fast PCRÀÇ ±¸ÇöÀ» À§ÇÏ¿© Bio-Rad¿¡¼´Â ƯÇã ¹ÞÀº ½Å°³³äÀÇ Mass-reduced ¡°honey-comb¡± blockÀ» Ãâ½ÃÇÏ¿´½À´Ï´Ù. |