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[¹Ì»ç¿ëǰ]Real-Time PCR CFX96 Detection System  
Á¦Á¶»ç : Bio-Rad
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[¹Ì»ç¿ëǰ] Real-time PCR CFX - 96

* Á¦Á¶»ç : Bio-Rad
*
¸ðµ¨¸í : CFX - 96   1set
*
»óÅ : ½Åǰ¼öÁØÀÓ

* C1000 Touch Thermal Cycler ÀÇ High performance ÀÔ´Ï´Ù

* MJ Reasearch »çÀÇ ¶Ù¾î³­ ±â¼ú·Â
* Realtime qPCR
ÀåºñÀÇ »õ·Î¿î ÆÐ·¯´ÙÀÓ
* C1000 Thermal Cycler
ÀÇ High performance
* 6
ä³Î Optic system
* Calibration
ÀÌ °øÁ¤°úÁ¤¿¡¼­ ¸ðµÎ ¿Ï·áµÇ¾î ¼Õ½±°Ô ½ÇÇè°úÁ¤ set up
* One-color
ºÎÅÍ 5-color multiplexing
*
¹ÝÀÀÀÌ ³¡³­ ÈÄ, ¾Ë¸² ¹× µ¥ÀÌÅÍ ÀÚµ¿Àü¼Û±â´É

CFX96 Real-Time PCR Detection System

  • MJ Research»çÀÇ ¶Ù¾î³­ ±â¼ú·Â ±×´ë·Î!
  • Realtime qPCR ÀåºñÀÇ »õ·Î¿î ÆÐ·¯´ÙÀÓ
  • C1000 Thermal cyclerÀÇ High-performance
  • 6ä³Î optic system
  • ReThink Realtime PCR
  • CalibrationÀÌ °øÁ¤°úÁ¤¿¡¼­ ¸ðµÎ ¿Ï·áµÇ¾î ¼Õ½±°Ô ½ÇÇè°úÁ¤ setup
  • ¼ö¸í°ú ¾ÈÁ¤¼ºÀÌ º¸ÀåµÈ LED ÀÇ »ç¿ë ¹× photodiodeÀÇ Á¶ÇÕ°ú Bio-Rad¸¸ÀÇ ±¸Á¶ ¼³°è¸¦ ¹ÙÅÁÀ¸·Î ÃÖ°íÀÇ °¨µµ¸¦ º¸¿©ÁÜ
  • ÇѹøÀÇ ¹ÝÀÀÀ¸·Î ÃÖÀûÀÇ Á¶°Ç¼öÀÔÀ» À§ÇÑ Tnermal Gradient
  • SybrGreen I  »ç¿ëÀÚ¸¦ À§ÇÑ One-colorfestºÎÅÍ 5-color multiplexing ±îÁö ´Ù¾çÇÑ ½ºÄµ¸ðµå Àû¿ë °¡´É
  • °£ÆíÇÑ Gene expression ºÐ¼® ÅøÀ» Á¦°øÇÏ´Â CFXmanager software
  • ÇÑ ´ëÀÇ ÄÄÇ»ÅÍ·Î ÃÖ´ë 4´ëÀÇ CFX96Àåºñ¸¦ µ¿½Ã ÄÁÆ®·Ñ
  • ¹ÝÀÀÀÌ ³¡³­ ÈÄ, »ç¿ëÀÚ¿¡°Ô ½ÇÇè Á¾·áÀÇ ¾Ë¸² ¹× µ¥ÀÌÅÍ ÀÚµ¿ Àü¼Û±â´É±îÁö  

 

Thermal Cycler

Base thermal cycler

C1000

Max ramp rate

5¡ÆC/sec

Ave ramp rate

3.3¡ÆC/sec

Heating and cooling method

Peltier

Lid

Heats up to 105¡ÆC

Temperature

 

Range

0–100¡ÆC

Accuracy

¡¾0.2¡ÆC within programmed target at 90¡ÆC

Uniformity

¡¾0.4¡ÆC within 10 sec of arrival at 90¡ÆC

Gradient

Yes

Operational range

30–100¡ÆC

Programmable span

1–24¡ÆC

Optical Detection

Excitation

6 filtered LEDs

Detection

6 filtered photodiodes

Range of excitation/emission
wavelengths

450–730 nm

Sensitivity

Detects one copy of genomic DNA

Dynamic range

10 orders of magnitude

Scan time — all channels

12 sec

Scan time — fast
single channel

3 sec

Software

Operating systems

Windows XP Professional, Windows Vista Professional

Multiplex analysis

Up to 5 targets per well

Data analysis modes

PCR quantitation with standard curve and melt-curve analyses; gene expression analysis by relative quantity (¥ÄCT) or normalized expression (¥Ä¥ÄCT) with multiple reference genes and reaction efficiencies; multiple file gene expression analysis; allelic discrimination and end-point analyses

Data export

Save, copy, and print all graphs and spreadsheets from right-click menu. Export results to Microsoft Excel. Copy and paste directly into Microsoft Excel, Word, or PowerPoint files.
Customizable reports containing run settings, data graphs, and spreadsheets can be directly printed or saved as PDF files.

System

Licensed for real-time PCR

Yes

Sample capacity

96 wells

Sample size

1–50 µl (recommend 10–25 µl)

Communications

USB 2.0

Electrical approvals

IEC, CE

Dimensions
(W x D x H)

33 x 46 x 36 cm (13 x 18 x 14")

Weight

21.4 kg (47 lb)

Channel

Excitation (nm)

Detection (nm)

Calibrated Fluorophores

1

450-490

515-530

 FAM¢â, SYBR Green I¢â

2

515-535

560-580

 VIC¢ç, HEX¢â, TET¢â, Cal Gold 540¢â

3

560-590

610-650

ROX¢â, TEXAS RED¢ç, Cal Red 610¢â

4

620-650

675-690

CY5, Quasar 670¢â

5

672-684

705-730

Quasar 705¢â

6

450-490

560-580

None (accommodates FRET Chemistry)

CFX96 filters and factory calibrated fluorophores

 

 

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°¢ ä³Î°£¿¡ ÀÚÁÖ »ç¿ëµÇ´Â Çü±¤dyeµéÀ»CFX96¿¡¼­ detectionÇÑ °ªÀÔ´Ï´Ù. ä³Î °£ Çü±¤ °£¼·ÀÌ °ÅÀÇ ¾øÀ½À» º¸¿©ÁÖ´Â µ¥ÀÌÅÍÀÔ´Ï´Ù.

Accurate quantitation is accomplished across a large dynamic range.  Plasmid template was diluted from 1010 copies down to 1 copy and amplified using iQ supermix in the presence of a FAM-labeled probe. Data are presented for 6 replicates per dilution.

 

 

CFX96ÀÇ High performance 1 : Linearity and Uniformity

img4.gif

1010°³ºÎÅÍ 1°³±îÁö plasmid DNA¸¦ 10¹è ´ÜÀ§·Î Èñ¼®ÇÏ¿© template·Î »ç¿ëÇϰí, FAM-labeled probe¸¦ ÀÌ¿ëÇÏ¿© detectionÇÑ °á°úÀÌ´Ù. °¢ dilution-factor³»¿¡¼­´Â 6¹ø ¹Ýº¹½ÇÇèÀ» ÇÏ¿´´Ù. CFX96À» »ç¿ëÇϸé ÃÖ°íÀÇ Á¤·®Àû µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ÀÖÀ½À» º¸¿©ÁÖ´Â °á°úÀÌ´Ù.

 

 

CFX96ÀÇ High performance 2 : true 5 target multiplexing °á°ú

Linearity of five-target detection.: 108ºÎÅÍ 102°³ ±îÁö plasmid DNA¸¦ 10¹è ´ÜÀ§·Î Èñ¼®ÇÏ¿© template·Î »ç¿ëÇϰí, °¢°¢ÀÇ targetÀ» °¢±â ´Ù¸¥ Çü±¤¹°Áú·Î probe¸¦ ¸¸µé¾î (FAM/actin, Hex/GAPDH, Texas Red/Cyclophilin, Cy5/Tubulin and Quasar705/IL1-bB) Realtime qPCR standard curve ½ÇÇèÀ» ÇÑ µ¥ÀÌÅÍÀÌ´Ù. ¸ðµç standard curveÀÇ ¹ÝÀÀ È¿À²(reaction efficiency)Àº 97%~103% ¾ÈÀ¸·Î µé¾î¿ÈÀ» º¼ ¼ö ÀÖ´Ù. À̷μ­ CFX96ÀÇ ¶Ù¾î´Â ¹ÝÀÀ¼ºÀ» º¸¿©Áܰú µ¿½Ã¿¡ ½ÇÁ¦ 5 target multiplex °á°ú¸¦ ¸¸µé¾î ³¾ ¼ö ÀÖ´Â ÀåºñÀÓÀ» ÀÔÁõÇϰí ÀÖ´Ù.

img5.gif

 

Maximum VS Average Ramping Rate.


´Ü¼øÇÑ Spec. »ó¿¡¼­ÀÇ Ramping rateÀÇ ºü¸§Àº Áß¿äÄ¡ ¾Ê´Ù°í »ý°¢ÇÒ ¼ö ÀÖ½À´Ï´Ù. ¿ÀÈ÷·Á Avg. ramp rateÀÇ ºü¸§ÀÌ Fast PCR À» º¸´Ù È¿À²ÀûÀ¸·Î À¯µµÇÒ ¼ö ÀÖ½À´Ï´Ù. ¹ØÀÇ 3°³ ȸ»çÀÇ Fast PCR ±â±âÀÇ running time ºñ±³¸¦ º¸¸é ¸íÈ®È÷ ±× Á߿伺À» ÀÌÇØÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ º¸´Ù Á¤È®Çϰí ÈǸ¢ÇÑ PCR performance¸¦ À§Çؼ­´Â ÁöÁ¤ ¿Âµµ¿¡¼­ÀÇ ¿Âµµ À¯Áö¸¸Å­À̳ª heating & cooling ½ÃÀÇ ¿Âµµ ±ÕÀϼº(uniformity)ÀÌ Áß¿äÇÏ´Ù°í ÇÒ ¼ö ÀÖ°Ú½À´Ï´Ù.

 

1000 series

  • 5C/sec max ramp rate
  • Accuracy:  +/- 0.2oC, 10 sec
  • Uniformity: +/- 0.4oC, 10 sec

 

   
 

Aȍ

  • 5.5¡ÆC/sec max ramp rate
  • Accuracy:  +/- 0.25oC, 3 mins
  • Uniformity: +/- 0.5oC, 30 sec

Eȍ

  • 6¡ÆC/sec max ramp rate
  • Accuracy:  +/- 0.2oC
  • Uniformity: +/- 0.4oC, ? min

*1000-series°¡ º¸¿©ÁÖ´Â ÁöÁ¤¿Âµµ·ÎÀÇ ºü¸¥ µµÂø ¹× ¶Ù¾î³­ heating & cooling ½ÃÀÇ uniformity

*ÀÌ¿Í °°ÀÌ ¶Ù¾î³­ Uniformity ¹× fast PCRÀÇ ±¸ÇöÀ» À§ÇÏ¿© Bio-Rad¿¡¼­´Â ƯÇã ¹ÞÀº ½Å°³³äÀÇ Mass-reduced ¡°honey-comb¡± blockÀ» Ãâ½ÃÇÏ¿´½À´Ï´Ù.

 

 

 
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